Proteomics and Human Proteome
From bench to bedside
10-13 February, 2009
Pamplona, Spain

Proteomics and Human Proteome
From bench to bedside
10-13 February, 2009
Pamplona, Spain

SEPROT LAHUPO UNAV CIMA

Wellcome and Opening Lecture

Wellcome
Dr. Luis Montuenga, Dr. Fernando Corrales
Wellcome and Prof. Michael Karas presentation
Dr. Juan J. Calvete
Good News for MALDI - The Advent of Second Generation Matrices
Prof. Michael Karas, Johann Wolfgang Goethe University

PRE-CONGRESS EDUCATIONAL DAY

Introduction to the course
(Dr. Juan Pablo Albar, CNB)
Basic concepts, sample preparation and separation technologies
(Dr. Juan Pablo Albar, CNB)
How to interpret a MS and a MS/MS spectra
(Dr. Joaquin Abián, CSIC-UAB)
SILAC (Stable Isotope Labeling with Amino acids in Cell culture) in differential proteomics
(Dr. Miguel Marcilla, CNB).
Non-isobaric isotope chemical labeling in differential proteomics: use of ICPL
(Dr. Alberto Paradela, CNB).
High-throughput quantitative proteomics by stable 18O isotope labeling
(Dra.Estefanía Nuñez and Dra. Elena Bonzón,CBMSO)
High-throughput quantitative proteomics by stable 18O isotope labeling
(Dra.Estefanía Nuñez and Dra. Elena Bonzón,CBMSO)
Label-free quantitative proteomics: advantages and disadvantages
(Dra. Miren Josu Omaetxeberria or Dr. Kerman Aloria, UPV-EHU)
Isobaric isotope chemical labeling: iTRAQ
(Dr Antonio Serna, Applied Biosystems)
  Mass Spectrometry Imaging Session - Overview and applications of tissue proteomics
(Dr. Garry Corthals, Turku Center for Biotechnoly,Turku, Finland)
High-resolution MS imaging and data analysis
(Dr. Ron Heeren, FOM Institute for Atomic and Molecular Physics, Amsterdam, The Netherlands.)
Ultra-flex Family, FlexImaging software and spray station
(Sören-Oliver Deininger, Bruker Daltonics)
MALDI-SYNAPT mass spectrometry imaging
(Dr. McDowell, Waters Corporation)
MS imaging by MALDI LTQ XL y MALDI LTQ-Orbitrap: The importance of MS3 and dynamic range.
( Dra Michaela Scigelova, Thermo Fisher Scientific)

Quantitative proteomics

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) as a Tool in Cancer Research
Dr. José Luis Luque. Spanish National Cancer Research Center
Isotope-Coded Protein Labelling (ICPL) as a tool for the quantitative analysis of a bacterial proteome
Dr. Alberto Paradela. Biotechnology National Center
Peptide fractionation for proteomic studies
Dr. Gabriel Padrón. Center for Genetic Engineering and Biotechnology, La Havana, Cuba
Metastasis-associated C4.4A, a GPI-anchored protein cleaved by ADAM10 and ADAM17
Francesc Canals. Vall d’Hebron University Hospital
Label-free quantification based on data independent acquisition mass spectrometry
Miren Josu Omaetxebarria. University of the Basque Country
Identification and Quantification of proteins from Methylophaga Thiooxidans and Methylocella Silvestris using label-free LC/MS
Joanne B. Connolly. Waters

Posttranslationals Modifications

Proteomic analysis of S-nitrosated proteins
Dr. Antonio Rodriguez Ariza. Reina Sofia University Hospital of Cordoba
Understanding by proteomics the cellular trafficking defect of a disease associated mutant protein
Dra. Deborah Penque. Instituto nacional de Saudé Dr. Ricardo Jorge, Lisboa.
Quantitative analysis of protein glycation in clinical samples
Feliciano Priego, University Medical Center, Geneva, Switzerland
New microfluidic chip targeting phosphoproteomes
Dayin Lin, Agilent Technologies

HUPO Initiatives

Liver
The Insight into the Human Liver Proteome

Prof. Fuchu He. Fudan University, Shanghai, China
Brain
Characterisation of the Autoimmune Antibody Repertoire of Parkinson’s Disease Patients by Systematic Screening of Protein Arrays

Prof. Helmut E. Meyer. Medizinisches Proteom-Center, Ruhr-Universität Bochum, Germany
PSI
Annotating the Human Proteome

Prof. Rolf Apweiler. EMBL/EBI, Hinxton, UK
ETD and CID Fragmentation
Prof. Henrik Molina

Bioinformatics in Proteomics

Application of a novel statistical model for quantitative proteomics by 18O labeling to the study of VEGF-induced angiogenesis in vascular endothelial cells
Pablo Martínez-Acedo. Severo Ochoa Molecular Biology Center
Properties of Average Score Distributions of SEQUEST: The Probability Ratio method
Pedro Navarro. Severo Ochoa Molecular Biology Center
Storing, reporting and comparing proteomics experiments using the miape generator tool
Salvador Martínez, ProteoRed – National Center for Biotechnology-CSIC
Proteopathogen, a protein database to study host-pathogen interaction
Vital Vialas, Complutense University of Madrid
HaloLink™ Protein Arrays for Functional Analysis of Proteins
Patricia Bresnahan, Promega Biotech Ibérica

Protein Interactions and Protein Arrays

Real-time and Label-free Biomolecular Interactions Analysis using Self-assembled Protein Microarrays and Surface Plasmon Resonance Imaging
Dr. Manuel Fuentes. Harvard Institute of Proteomics, Harvard Medical School.
Deciphering the interactome of p8, a protein related to tumor progression
Dra. Silvia Moreno. Universidad de Buenos Aires, Argentina
High-Mass MALDI ToF Mass Spectrometry and Chemical Cross-linking for Interaction Analysis
Alexis Nazabal, Swiss Federal Institute of Chemistry
Lectin-sugar interactions deciphered by SPR-MS and CREDEX-MS
Carmen Jimenez, Pompeu Fabra University
Monitoring in vivo protein-protein interactions by coupling bimolecular fluoresecence complementation (BIFC) and flow cytometry
Montse Morell. Universidad Autónoma de Barcelona

Human Proteomics and Biomarkers

Proteomics in obesity research
Dra. Silvia Barceló. Instituto Aragonés de Ciencias de la Salud
Using proteomics to unravel the molecular pathway of sparc-mediated tumorigenicity
Dra. Andrea Llera. Fundación Instituto Leloir, Buenos Aires, Argentina
Detection of novel biomarkers of liver cirrosis by proteomic analysis
Prof. Helmut E. Meyer. Medizinisches Proteom-Center, Ruhr-Universität Bochum, Germany
Identification of replication-competent HSV-1 Cgal+ strain signalling targets in human hepatoma cells by functional organelle proteomics
Enrique Santamaría, Centre for Applied Medical Research (CIMA), University of Navarra
BioCore study (“Biomarkers of Coronary Events”): from sampling to discovery of plasma biomarkers by SELDI-TOF MS and 2DE
Olivier Meilhac. Hôpital Xavier Bichat - PARIS. Bio-Rad

Seprot Awards

Seprot Award Session
Cyclostreptin binds covalently to microtubule pores and lumenal taxoid binding sites

Enrique Calvo, Spanish National Centre for Cardiovascular Research

Animal, Plant and Microbial Proteomics

Top-down and bottom-up quantitative proteomic approaches to characterize the development of grape berry tissues
Dr. Roque Bru. University of Alicante
Proteomic identification of s-nitrosylated proteins in arabidopsis thaliana in response to pathogen infection
Dra. Ana María Maldonado. University of Cordoba
Using DM43 and DM64, two antitoxins from Didelphidae, to study the snake venom sub-proteomes
Dr. Jonas Perales. Oswaldo Cruz Foundation, Brazil
Combined proteomic and transcriptomic analysis identifies differentially expressed pathways associated to Pinus Radiata needle maturation
Luis Valledor, Univesity of Oviedo
Quantitative proteomics analysis of lymph nodes from pigs infected by porcine circovirus type 2 (PCV2) by 2-DE, 18O/16O labeling and linear ion trap mass spectrometry
María Ramirez, University of Córdoba
Intelligent Use of Retention Time for Higher Order Multiple Reaction Monitoring Multiplexing
Antonio Serna, Applied Biosystems

Closing Lecture

Closing presentation
Prof. Fernando Corrales
Never give up your dreams
Prof. Peter Roepstorff. University of Southern Denmark
Acknowledgment
Prof. Fernando Corrales