Proteomics and Human Proteome
From bench to bedside
10-13 February, 2009
Pamplona, Spain

SEPROT LAHUPO UNAV CIMA
Precongress day

PRE-CONGRESS EDUCATIONAL DAY

FROM BASIC TO CHALLENGING PROTEOMICS TECHNOLOGIES

 

Educational Day, 10th of February

Program (provisional)

 

Chairs Dr. Juan Pablo Albar and Dr. Alberto Paradela

08:45-09:00 Introduction to the course
(Dr. Juan Pablo Albar, CNB)
Fundamental Proteomics Session
09:00-09:40Basic concepts, sample preparation and separation technologies
(Dr. Juan Pablo Albar, CNB-CSIC)
09:40-10:20How to interpret a MS and a MS/MS spectra
(Dr. Joaquin Abián, CSIC-UAB)        
Differential Quantitative Proteomics Session
10:20-11:10SILAC (Stable Isotope Labeling with Amino acids in Cell culture) in differential proteomics
(Dr. Miguel Marcilla, CNB).
11:10-11:30Coffee break

11:30-12:10Non-isobaric isotope chemical labeling in differential proteomics: use of ICPL
(Dr. Alberto Paradela, CNB).

12:10-12:50High-throughput quantitative proteomics by stable 18O isotope labeling
(Dra.Estefanía Nuñez and Dra. Elena Bonzón,CBMSO).
12:50-13:30Label-free quantitative proteomics: advantages and  disadvantages
(Dra. Miren Josu Omaetxeberria or Dr. Kerman Aloria, UPV-EHU).
13:30-14:30 Lunch time
14:30-15:10Isobaric isotope chemical labeling: iTRAQ
(Dr Antonio Serna,  Applied Biosystems).
Mass Spectrometry Imaging Session

15:10-15:45Overview and applications of tissue proteomics
(Dr. Garry   Corthals, Turku Center for Biotechnoly,Turku, Finland)
15:45-16:20High-resolution MS imaging and data analysis
(Dr. Ron Heeren, FOM Institute for Atomic and Molecular Physics, Amsterdam, The Netherlands.).

16:20-16:40Coffee break
16:20-17:15Ultra-flex Family, FlexImaging software and spray station
(Sören-Oliver Deininger, Bruker Daltonics).
17:15-17:50 MALDI-SYNAPT mass spectrometry imaging
(Dr James Langridge, Water Corporation)
17:50-18:25 MS imaging by MALDI LTQ XL y MALDI LTQ-Orbitrap: The importance of MS3 and dynamic range.
( Dra Michaela Scigelova, Thermo Fisher Scientific)
18:30-18:45Concluding remarks
Abbreviatures:

CNBCentro Nacional de Biotecnología, Madrid, Spain.
CBMSOCentro de Biología Molecular Severo Ochoa, Madrid, Spain.
UPV-EHUUniversidad del Pais Vasco-Euskal Herriko Unibertsitatea, Bilbao, Spain.