PRE-CONGRESS EDUCATIONAL DAY
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FROM BASIC
TO CHALLENGING PROTEOMICS TECHNOLOGIES
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Educational Day, 10th of
February
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Program (provisional)
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Chairs Dr. Juan Pablo Albar and Dr. Alberto Paradela
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08:45-09:00 | Introduction to the course (Dr. Juan Pablo Albar, CNB)
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Fundamental
Proteomics Session
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09:00-09:40 | Basic
concepts, sample preparation and separation technologies (Dr. Juan Pablo Albar, CNB-CSIC)
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09:40-10:20 | How
to interpret a MS and a MS/MS spectra (Dr. Joaquin Abián, CSIC-UAB)
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Differential Quantitative Proteomics
Session
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10:20-11:10 | SILAC (Stable Isotope Labeling with Amino acids
in Cell culture) in differential proteomics (Dr. Miguel Marcilla, CNB).
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11:10-11:30 | Coffee break
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11:30-12:10 | Non-isobaric isotope chemical labeling in
differential proteomics: use of ICPL (Dr. Alberto Paradela, CNB).
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12:10-12:50 | High-throughput quantitative proteomics by stable 18O isotope labeling (Dra.Estefanía Nuñez and Dra. Elena Bonzón,CBMSO).
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12:50-13:30 | Label-free quantitative proteomics: advantages and disadvantages (Dra. Miren Josu Omaetxeberria or Dr. Kerman Aloria, UPV-EHU).
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13:30-14:30 | Lunch time
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14:30-15:10 | Isobaric isotope chemical labeling: iTRAQ (Dr Antonio Serna, Applied Biosystems).
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Mass Spectrometry Imaging Session
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15:10-15:45 | Overview and applications of tissue proteomics
(Dr. Garry Corthals, Turku Center for Biotechnoly,Turku, Finland)
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15:45-16:20 | High-resolution MS imaging and data analysis (Dr. Ron Heeren, FOM Institute for Atomic and Molecular Physics, Amsterdam, The Netherlands.).
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16:20-16:40 | Coffee break
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16:20-17:15 | Ultra-flex Family, FlexImaging software and spray station (Sören-Oliver Deininger, Bruker Daltonics).
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17:15-17:50 | MALDI-SYNAPT
mass spectrometry imaging (Dr James Langridge, Water Corporation)
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17:50-18:25 | MS imaging by MALDI LTQ XL y MALDI LTQ-Orbitrap: The importance of MS3 and dynamic range. ( Dra Michaela Scigelova, Thermo Fisher Scientific)
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18:30-18:45 | Concluding remarks
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Abbreviatures:
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CNB | Centro
Nacional de Biotecnología, Madrid, Spain.
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CBMSO | Centro
de Biología Molecular Severo Ochoa, Madrid, Spain.
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UPV-EHU | Universidad
del Pais Vasco-Euskal Herriko Unibertsitatea, Bilbao, Spain.
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